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Spirit Blue Agar
[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]
Spirit Blue Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................20.0g
Pancreatic digest of casein................................10.0g
Yeast extract........................................................5.0g
Spirit Blue.........................................................0.15g
Lipoidal emulsion ......................................... 30.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Lipoidal Emulsion:
Composition per 500.0mL:
Tween 80......................................................... 1.0mL
Cottonseed oil or olive oil........................... 100.0mL
Preparation of Lipoidal Emulsion: Add Tween
80 to 400.0mL of warm distilled/deionized water.
Mix thoroughly. Add 100.0mL of cottonseed or olive
oil. Emulsify in a blender. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, ex
cept lipoidal emulsion, to distilled/deionized water
and bring volume to 970.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptical
ly add 30.0mL of sterile lipoidal emulsion. Mix thor
oughly. Pour into sterile Petri dishes while shaking
flask to keep emulsion dispersed.
Use: For the detection, enumeration, and study of li
polytic microorganisms.