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Spray’s Fermentation Medium (BAM M141)

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

Spray’s Fermentation Medium

(BAM M141)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Neopeptone .......................................................10.0g

Pancreatic digest of casein................................10.0g

Agar ....................................................................2.0g

Sodium thioglycolate ......................................0.025g

Carbohydrate solution................................. 110.0mL

pH 7.4 ± 0.1 at 25°C

Carbohydrate Solution:

Composition per 200.0mL:

Carbohydrate.....................................................20.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 200.0mL. Glucose or glycerol may be

used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept agar and carbohydrate solution, to distilled/

deionized water and bring volume to 990.0mL. Mix

thoroughly. Adjust pH to 7.4. Add agar. Gently heat

and bring to boiling. Distribute into tubes in 9.0mL

volumes. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Immediately prior to use, heat

tubes in a boiling water bath for 10 min. Cool to

45°C. Aseptically add 1.0mL of sterile carbohydrate

solution. Mix thoroughly.

Use: For the cultivation and differentiation of

Clostridium perfringens based on carbohydrate fer

mentation patterns.