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SS Deoxycholate Agar (Salmonella-Shigella Deoxycholate Agar) (SSDC)

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

SS Deoxycholate Agar

(Salmonella-Shigella

Deoxycholate Agar)

(SSDC)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

Lactose..............................................................10.0g

Sodium deoxycholate........................................10.0g

Bile salts..............................................................8.5g

Na2S2O3 ..............................................................8.5g

Sodium citrate.....................................................8.5g

Beef extract.........................................................5.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue .............................2.5g

CaCl2·2H2O ........................................................1.0g

Ferric citrate........................................................1.0g

Neutral Red.....................................................0.025g

Brilliant Green ...............................................0.33mg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes in 20.0mL volumes. Allow

the surface of the plates to dry before inoculation.

Use: For the isolation and cultivation of Yersinia en

terocolitica from foods.