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Standard Methods Caseinate Agar

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Sodium caseinate ..............................................10.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................2.5g

Glucose ...............................................................1.0g

Trisodium citrate solution.................................. 1.0L

CaCl2·2H2O (1M solution)............................ 20.0mL

Trisodium Citrate Solution:

Composition per liter:

Trisodium citrate·2H2O.....................................4.41g

Preparation of Trisodium Citrate Solution:

Add trisodium citrate·2H2O to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add yeast extract, pan

creatic digest of casein, glucose, and agar to 500.0mL

of the trisodium citrate solution. Mix thoroughly. Gen

tly heat until boiling. Add the remaining components.

Bring volume to 1.0L with trisodium citrate solution.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Pour into sterile Petri dishes.

Use: For the cultivation of proteolytic bacteria from

foods.