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Streptococcal Growth Medium

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion......................500.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Glucose ...............................................................1.0g

Bromcresol Purple solution ............................ 1.0mL

pH 7.4 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ............................................0.16g

Ethanol (95% solution) ................................. 10.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 10.0mL of ethanol. Mix

thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes in 5.0mL vol

umes. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Streptococcus species and

other Gram-positive cocci. Growth in this medium

turns the indicator yellow and the solution turbid.