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Streptococcus Selective Medium

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone.................................................23.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Horse blood, defibrinated ............................. 50.0mL

Antibiotic inhibitor ....................................... 10.0mL

pH 7.3± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Antibiotic Inhibitor:

Composition per 10.0mL:

Colistin sulfate...............................................10.0mg

Oxolinic acid....................................................5.0mg

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept horse blood and antibiotic inhibitor, to distilled/

deionized water and bring volume to 940.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile horse blood and

sterile antibiotic inhibitor. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of streptococci from

clinical specimens or foodstuffs.