培养基配方

您现在的位置：网站首页 >> 培养基配方

Sucrose Yeast Extract Medium

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................20.0g

Yeast extract........................................................4.0g

K2HPO4...............................................................2.5g

MgSO4·7H2O ......................................................1.0g

Trace elements solution .................................. 4.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

ZnSO4·7H2O .....................................................18.0g

FeSO4·7H2O........................................................9.0g

MnSO4·4H2O......................................................3.0g

CoCl2·6H2O ........................................................0.9g

CuSO4·5H2O.......................................................0.8g

H2SO4 (Concentrated) .................................... 5.0mL

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a variety of bacteria that

can utilize sucrose as carbon source.