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Tetrathionate Broth (BAM M145)

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tetrathionate Broth

(BAM M145)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1030.0mL:

Tetrathionate broth base..................................... 1.0L

Iodine-potassium iodide solution.................. 20.0mL

Brilliant Green solution ................................ 10.0mL

pH 8.4 ± 0.2 at 25°C

Tetrathionate Broth Base:

Composition per liter:

Na2S2O3·5H2O ..................................................30.0g

CaCO3 ...............................................................10.0g

Polypeptone ........................................................5.0g

Bile salts..............................................................1.0g

Preparation of Tetrathionate Broth Base:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. A slight precipitate will remain.

Do not autoclave. Cool to 25°C. Store at 4°C.

Iodine–Potassium Iodide Solution:

Composition per 20.0mL:

Iodine, resublimed ..............................................6.0g

KI ........................................................................5.0g

Preparation of Iodine–Potassium Iodide So

lution: Add KI to 5.0mL of sterile distilled/deion

ized water. Mix thoroughly. Add iodine. Mix

thoroughly. Bring volume to 20.0mL with sterile dis

tilled/deionized water.

Brilliant Green Solution:

Composition per 100.0mL:

Brilliant Green ....................................................0.1g

Preparation of Brilliant Green Solution: Add

Brilliant Green to sterile distilled/deionized water.

Mix thoroughly.

Preparation of Medium: Combine 1.0L of

tetrathionate broth base, 20.0mL of iodine-potassium

iodide solution, and 10.0mL of Brilliant Green solu

tion. Mix thoroughly. Aseptically distribute into

tubes in 10.0mL volumes. Do not heat medium after

it has been mixed.

Use: For the selective isolation and cultivation of

Salmonella species from foods.