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Tetrathionate Broth with Novobiocin

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ............................................................38.0g

CaCO3 ...............................................................25.0g

Casein/meat peptone (50/50) ............................18.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

D–Mannitol .........................................................0.5g

Glucose ...............................................................0.5g

Sodium deoxycholate..........................................0.5g

Brilliant Green ..................................................0.01g

Novobiocin.......................................................4.0mg

Iodine solution .............................................. 40.0mL

pH 7.5–7.8 at 25°C

Iodine Solution:

Composition per 40.0mL:

KI ........................................................................8.0g

Iodine ..................................................................5.0g

Preparation of Iodine Solution: Add iodine and

KI to distilled/deionized water and bring volume to

20.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept iodine solution, to distilled/deionized water and

bring volume to 960.0mL. Mix thoroughly. Gently

heat and bring to boiling. Do not autoclave. Cool to

40°C. Add 40.0mL of iodine solution. Mix thorough

ly. Distribute into tubes in 10.0mL volumes. Use me

dium the same day it is prepared.

Use: For the isolation of Salmonella species, except

Salmonella typhi, and Arizona species. Novobiocin

suppresses the growth of Proteus species.