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Tetrazolium Thallium Glucose Agar

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................14.0g

Beef extract.......................................................10.0g

Peptone .............................................................10.0g

Glucose solution ......................................... 100.0mL

2,3,5-Triphenyltetrazolium·HCl solution...... 10.0mL

Thallous acetate solution .............................. 10.0mL

2,3,5-Triphenyltetrazolium·HCl Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium·HCl .........................0.1g

Preparation of 2,3,5-Triphenyltetrazolium

HCl Solution: Add 2,3,5-triphenyltetrazolium·HCl

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Autoclave for 7 min at 15

psi pressure–121°C.

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................10.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Thallous Acetate Solution:

Composition per 10.0mL:

Thallous acetate ..................................................1.0g

Preparation of Thallous Acetate Solution:

Add thallous acetate to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components—ex

cept glucose solution, 2,3,5-triphenyltetrazolium·HCl

solution, and thallous acetate solution—to distilled/

deionized water and bring volume to 880.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile glucose solution,

2,3,5-triphenyltetrazolium·HCl solution, and thallous

acetate solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation of Streptococcus species.