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Thioglycolate Medium (DSMZ Medium 530)

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium

(DSMZ Medium 530)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone from casein............................................5.0g

Meat extract ........................................................3.0g

Na-thioglycolate solution............................ 100.0mL

pH 5.5 ± 0.2 at 25°C

Thioglycolate Solution :

Composition per 100mL:

Na-Thioglycolate ................................................0.5g

Preparation of Thioglycolate Solution: Add

thioglycolate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Warm to 50°C.

Preparation of Medium: Add components, ex

cept thioglycolate solution to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add 100.0mL warm thioglycolate solution. Ad

just pH to 5.5. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and maintenance of various

anaerobic bacteria.