培养基配方

您现在的位置：网站首页 >> 培养基配方

Tomato Juice Medium

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Tomato Juice Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Polypeptone ........................................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................0.5g

CaCl2·2H2O.....................................................0.125g

KCl..................................................................0.125g

MgSO4·7H2O ..................................................0.125g

NaCl................................................................0.125g

Bromcresol Green .............................................0.03g

MnSO4·4H2O ...................................................3.0mg

Tomato juice, canned .................................. 150.0mL

Cycloheximide solution ................................ 10.0mL

pH 5.0 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Preparation of Cycloheximide Solution: Add

cycloheximide to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept cycloheximide solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile cycloheximide solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation and cultivation of Lactobacilli

from wine.