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Tributyrin Agar

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Tributyrin Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Tributyrin (glyceryl tributyrate)........................10.0g

Peptone ...............................................................5.0g

Yeast extract........................................................3.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es.

Use: For the cultivation and enumeration of lipolytic

fungi and bacteria, especially Staphylococcus spe

cies, Flavobacterium species, Clostridium species,

and Pseudomonas species from butter. Lipolytic bac

teria appear as colonies surrounded by a clear zone.