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Triple Sugar Iron Agar (TSI Agar)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Triple Sugar Iron Agar

(TSI Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................20.0g

Agar ..................................................................12.0g

Lactose..............................................................10.0g

Sucrose..............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Ferric citrate........................................................0.3g

Na2S2O3 ..............................................................0.3g

Phenol Red......................................................0.025g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Allow tubes to cool in a

slanted position to form a 1.0-inch butt.

Use: For the differentiation of members of the Enter

obacteriaceae based on their fermentation of lactose,

sucrose, and glucose and the production of H2S.