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Tryptic Soy Agar with 0.6% Yeast Extract

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptic Soy Agar

with 0.6% Yeast Extract

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................15.0g

Yeast extract........................................................6.0g

Pancreatic digest of soybean meal......................5.0g

NaCl....................................................................5.0g

pH 7.0–7.5 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the isolation and cultivation of Listeria

monocytogenes from foods.