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Trypticase Novobiocin Broth (TN Broth) (BAM M150)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Novobiocin Broth

(TN Broth)

(BAM M150)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................17.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

K2HPO4...............................................................2.5g

Glucose ...............................................................2.5g

Bile salts No. 3....................................................1.5g

K2HPO4...............................................................1.5g

Novobiocin solution...................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin........................................................0.02g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile novobiocin solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of verotoxin-producing Es

cherichia coli.