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Trypticase Peptone Glucose Yeast Extract Broth, Buffered

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Peptone Glucose

Yeast Extract Broth, Buffered

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................50.0g

Yeast extract......................................................20.0g

Na2HPO4.............................................................5.0g

Peptone ...............................................................5.0g

Glucose ...............................................................4.0g

Sodium thioglycolate..........................................1.0g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat until dissolved. Adjust

pH to 7.3. Distribute into tubes in 15.0mL volumes.

Autoclave for 8 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Clostridium

perfringens from foods.