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Trypticase Soy Agar with Lecithin and Polysorbate 80 (Microbial Content Test Agar)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Soy Agar

with Lecithin and Polysorbate 80

(Microbial Content Test Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Agar ..................................................................15.0g

Papaic digest of soybean meal............................5.0g

NaCl....................................................................5.0g

Polysorbate 80 (Tween 80) .................................5.0g

Lecithin...............................................................0.7g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 13 psi pressure–118°C. Cool to 45°–50°C. Pour

into sterile Petri dishes in 17.0mL volumes or leave

in tubes.

Use: For the detection and enumeration of microor

ganisms in replicate plating techniques. Also used for

the detection and enumeration of microorganisms

present on surfaces of sanitary importance.