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Trypticase Soy Broth, Modified (mTSB) (BAM M156)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Soy Broth, Modified

(mTSB)

(BAM M156)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1000.2mL:

Pancreatic digest of casein................................17.0g

NaCl....................................................................5.0g

K2HPO4 ..............................................................4.0g

Papaic digest of soybean meal............................3.0g

Glucose ...............................................................2.5g

Bile salts No. 3....................................................1.5g

Novobiocin solution........................................ 0.2mL

pH 7.3 ± 0.2 at 25°C

Novobiocin Solution:

Composition per liter:

Novobiocin........................................................0.05g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile novobiocin solution. Mix thoroughly.

Aseptically distribute into sterile tubes.

Use: For the isolation and cultivation of Shigella

species from food.