培养基配方

您现在的位置：网站首页 >> 培养基配方

Tryptone Bile X-glucuronide Agar, Chromocult (Chromocult TBX) (Chromocult Tryptone Bile X-glucuronide Agar)

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Bile X-glucuronide Agar,

Chromocult

(Chromocult TBX)

(Chromocult Tryptone Bile

X-glucuronide Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ............................................................20.0g

Agar ..................................................................15.0g

Bile salts No. 3 ...................................................1.5g

X-β-D-glucuronide .........................................0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes.

Use: For the differentiation of E. coli from other

coliforms. The presence of the enzyme β-D-glucu

ronidase differentiates most E. coli spp. from other

coliforms. E. coli absorbs the chromogenic substrate

5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-β-D

glucuronide). The enzyme β-glucuronidase splits the

bond between the chromophore 5-bromo-4-chloro-3-

indolyl- and the β-D-glucuronide. E. coli colonies are

colored blue-green. Growth of accompanying Gram

positive flora is largely inhibited by the use of bile

salts. The prepared medium is clear and yellowish.