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Tryptone Phosphate Brain Heart Infusion Yeast Extract Agar (TPBY)

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Phosphate Brain Heart

Infusion Yeast Extract Agar

(TPBY)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Agar ..................................................................15.0g

NaCl..................................................................5.14g

K2HPO4...............................................................2.0g

KH2PO4...............................................................2.0g

Yeast extract........................................................1.0g

Oxgall..................................................................0.5g

Pancreatic digest of gelatin.................................0.4g

Brain heart, solids from infusion ......................0.16g

Peptic digest of animal tissue............................0.16g

Glucose .............................................................0.08g

Na2HPO4 ...........................................................0.06g

Tween 80......................................................... 1.5mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Adjust pH to 7.0. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation of coliform bacteria, such as

Escherichia coli, from foods.