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Tryptone Yeast Extract Agar (BAM M165)

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Yeast Extract Agar

(BAM M165)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Agar ....................................................................2.0g

Yeast extract........................................................1.0g

Bromcresol Purple ............................................0.04g

Carbohydrate solution................................. 100.0mL

pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate (glucose or mannitol for Staphylococ

cus aureus) to distilled/deionized water and bring

volume to 100.0mL. Glucose or mannitol may be

used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Adjust pH to 7.0. Gently heat and bring to boiling.

Distribute into tubes in 13.5mL volumes. Autoclave

for 20 min at 10 psi pressure–115°C. Cool to 45°–

50°C. Aseptically add 1.5mL of carbohydrate solu

tion to each tube. Mix thoroughly. Solidify agar

quickly by placing tubes in ice water.

Use: For the cultivation and differentiation of Sta

phylococcus aureus based on glucose and mannitol

fermentation. Bacteria that ferment the added carbo

hydrate turn the medium yellow.