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Tryptophan Broth

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptophan Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

L-Tryptophan.......................................................0.5g

NaCl....................................................................0.5g

KH2PO4.............................................................0.25g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Adjust pH to 7.4. Filter

sterilize. Aseptically distribute in 1.0mL volumes

into sterile screw-capped tubes.

Use: Used to differentiate bacteria based on indole

production. Indole is determined by the addition of

modified Kovacs reagent to cultures that have incu

bated for 18–24 hr. Formation of a red color in the

upper layer indicates indole formation.