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Tryptose Agar with Sheep Blood (ATCC Medium 546)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptose Agar with Sheep Blood

(ATCC Medium 546)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Sheep blood, defribinated .........................50-100mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept sheep blood to distilled/deionized water and

bring volume to 900-950mL. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile sheep blood. Mix

thoroughly. Pour into sterile Petri dishes in 17.0mL

volumes or distribute into sterile tubes.

Use: For the cultivation and maintenance of a wide

variety of fastidious microorganisms.