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Tryptose Sulfite Cycloserine Agar (TSC Agar) (BAM M169)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptose Sulfite Cycloserine Agar

(TSC Agar)

(BAM M169)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................15.0g

Agar ..................................................................14.0g

Beef extract.........................................................5.0g

Pancreatic digest of soybean meal......................5.0g

Yeast extract........................................................5.0g

Ferric ammonium citrate.....................................1.0g

Na2S2O5 ..............................................................1.0g

Egg yolk emulsion ........................................ 50.0mL

Cycloserine solution ..................................... 10.0mL

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Cycloserine Solution:

Composition per 10.0mL:

D-Cycloserine......................................................0.4g

Preparation of Cycloserine Solution: Add cy

closerine to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept cycloserine solution and egg yolk emulsion, to

distilled/deionized water and bring volume to

940.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile cy

closerine solution and egg yolk emulsion. Mix thor

oughly. Pour into sterile Petri dishes.

Use: For the presumptive identification and enumer

ation of Clostridium perfringens.