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Tryptose Sulfite Cycloserine Agar without Egg Yolk (TSC Agar without Egg Yolk)
[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]
Tryptose Sulfite Cycloserine Agar
without Egg Yolk
(TSC Agar without Egg Yolk)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Tryptose ............................................................15.0g
Agar ..................................................................14.0g
Beef extract.........................................................5.0g
Pancreatic digest of soybean meal......................5.0g
Yeast extract........................................................5.0g
Ferric ammonium citrate.....................................1.0g
Na2S2O5 ..............................................................1.0g
Cycloserine solution ..................................... 10.0mL
pH 7.6 ± 0.2 at 25°C
Cycloserine Solution:
Composition per 10.0mL:
D-Cycloserine......................................................0.4g
Preparation of Cycloserine Solution: Add cy
closerine to distilled/deionized water and bring vol
ume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept cycloserine solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptical
ly add sterile cycloserine solution. Mix thoroughly.
Pour into sterile Petri dishes.
Use: For the presumptive identification and enumer
ation of Clostridium perfringens.