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Universal Beer Agar

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

Universal Beer Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptonized milk ................................................15.0g

Agar ..................................................................12.0g

Yeast extract......................................................10.0g

Glucose .............................................................10.0g

Tomato juice solids .............................................7.0g

K2HPO4...............................................................0.5g

KH2PO4...............................................................0.5g

MgSO4·5H2O ......................................................0.2g

NaCl..................................................................0.01g

FeSO4·7H2O......................................................0.01g

MnSO4·H2O ......................................................0.01g

Beer............................................................. 250.0mL

pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components, ex

cept beer, to distilled/deionized water and bring vol

ume to 750.0mL. Mix thoroughly. Gently heat and

bring to boiling. Add beer. Mix thoroughly. Distrib

ute into tubes or flasks. Autoclave for 10 min at 15

psi pressure–121°C. Cool to 50°C. Aseptically add

250.0mL of beer without degassing. Pour into sterile

Petri dishes or leave in tubes.

Use: For the enumeration of contaminating bacteria

and yeasts encountered in wort and beer.