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Uric Acid Semisolid Agar for Clostridia

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ....................................................................2.0g

K2HPO4...............................................................4.0g

Uric acid..............................................................3.0g

Yeast extract........................................................1.0g

Sodium thioglycolate..........................................0.5g

MgSO4·7H2O......................................................0.1g

FeSO4·7H2O.....................................................5.0mg

Phenol Red (0.04% solution).......................... 1.0mL

pH 7.6-8.0 at 25°C

Preparation of Medium: Add components, ex

cept uric acid, to approximately 900.0mL of distilled/

deionized water. Mix thoroughly. Gently heat and

bring to boiling. Adjust pH to 7.6 with 1N NaOH.

Add the uric acid. Mix thoroughly. Adjust pH to 7.6.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of anaero

bic bacteria, such as Clostridium acidurici and

Clostridium cylindrosporum, that can utilize uric

acid as sole source of carbon and energy.