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UVM Listeria Enrichment Broth (University of Vermont Listeria Enrich ment Broth)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

UVM Listeria Enrichment Broth

(University of Vermont Listeria Enrich

ment Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Na2HPO4 .............................................................9.6g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

Beef extract.........................................................5.0g

Yeast extract........................................................5.0g

KH2PO4.............................................................1.35g

Esculin ................................................................1.0g

Nalidixic acid.................................................40.0mg

Acriflavine·HCl..............................................12.0mg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the selective isolation of Listeria monocyto

genes.