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Vibrio parahaemolyticus Agar (VP Agar)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio parahaemolyticus Agar

(VP Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

NaCl..................................................................20.0g

Sucrose..............................................................20.0g

Sodium citrate...................................................10.0g

Na2S2O3·5H2O ..................................................10.0g

Peptone .............................................................10.0g

Sodium taurocholate ...........................................5.0g

Yeast extract........................................................5.0g

Sodium lauryl sulfate..........................................0.2g

Bromthymol Blue .............................................0.04g

Thymol Blue .....................................................0.04g

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Pour into sterile Petri dishes.

Use: For the isolation, cultivation, enumeration,

and presumptive identification of coliforms in

milk, food, and other specimens of sanitary signif

icance. For the enumeration of bacteria in cheese,

especially Pseudomonas fragi, Pseudomonas vis

cosa, and Alcaligenes metalcaligenes. Sucrose-fer

menting bacteria appear as yellow colonies with

pale yellow peripheries. Sucrose-nonfermenting

bacteria appear as mucoid, green colonies with a

dark green center.