培养基配方

您现在的位置：网站首页 >> 培养基配方

Vibrio vulnificus Agar (VVA) (BAM M190)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Vibrio vulnificus Agar

(VVA)

(BAM M190)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Agar ..................................................................25.0g

Peptone..............................................................20.0g

Cellobiose solution ..................................... 100.0mL

Dye solution.................................................. 10.0mL

pH 8.2 ± 0.2 at 25°C

Dye Solution:

Composition per 100.0mL:

Bromthymol Blue ...............................................0.6g

Ethanol, 70%............................................... 100.0mL

Preparation of Dye Solution: Add Bromthymol

Blue to 100.0mL of 70% ethanol. Mix thoroughly.

Cellobiose Solution:

Composition per 100.0mL:

Cellobiose .........................................................10.0g

Preparation of Cellobiose Solution: Add cello

biose to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Gently heat while mix

ing to dissolve the cellobiose. Cool. Filter sterilize.

Preparation of Medium: Add components, ex

cept cellobiose solution, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat until dissolved. Adjust pH to 8.2. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add 100.0mL sterile cellobiose solution.

Mix thoroughly and pour into sterile Petri dishes. Fi

nal color of medium should be light blue.

Use: For the detection of Vibrio vulnificus from sea

foods.