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Vitamin B12 Assay Medium

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................40.0g

Sodium acetate..................................................20.0g

Vitamin assay casamino acids ..........................12.0g

Sorbitan monooleate complex ............................2.0g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.4g

DL-Tryptophan.....................................................0.2g

L-Cystine .............................................................0.2g

Adenine.............................................................0.02g

FeSO4................................................................0.02g

Guanine.............................................................0.02g

MnSO4·5H2O....................................................0.02g

NaCl..................................................................0.02g

Uracil ................................................................0.02g

Pyridoxine·HCl ................................................4.0mg

Niacin...............................................................2.0mg

Riboflavin ........................................................2.0mg

Thiamine·HCl ..................................................2.0mg

Xanthine...........................................................1.0mg

Calcium pantothenate ......................................200μg

p-Aminobenzoic acid.......................................200μg

Folic acid .........................................................100μg

Biotin .................................................................10μg

pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes in

5.0mL volumes. Add standard solution or test solu

tions to each tube. Adjust the volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of vitamin

B12 using Lactobacillus leichmannii as the test or

ganism.