培养基配方

您现在的位置：网站首页 >> 培养基配方

VRB Agar, Fluorocult Fluorocult VRB Agar

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

VRB Agar, Fluorocult

Fluorocult VRB Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.0g

Lactose..............................................................10.0g

Peptone from meat..............................................7.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

Bile salt mixture..................................................1.5g

4-Methylumbelliferyl-β-D-glucuronide ..............0.1g

Neutral Red.......................................................0.03g

Crystal Violet ..................................................0.002g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Heat in a boiling water bath or in

free flowing steam with frequent stirring until com

pletely dissolved. Do not boil for more than 2 min.

Do not autoclave. Do not overheat. Pour into sterile

Petri dishes. The plates are clear and dark red.

Use: For the detection and enumeration of coliform

bacteria, in particular E. coli. Crystal violet and bile

salts largely inhibit the growth of Gram-positive ac

companying bacterial flora. Lactose-positive colo

nies show a color change to red of the pH indicator.

E. coli colonies show a fluorescence under UV light.

Lactose-negative Enterobacteriaceae are colorless.

Lactose-positive colonies are red and often surround

ed by a turbid zone due to the precipitation of bile ac

ids. Light blue fluorescing colonies denote E. coli.