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Wesley Broth

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wesley Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................20.0g

Bicine (N,N-bis-2-[hydroxy

ethyl]glycine) buffer...................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.5g

Agar ....................................................................1.0g

FeSO4 ................................................................0.25g

Na2S2O5 ............................................................0.25g

Sodium pyruvate...............................................0.25g

Antibiotic solution ........................................ 10.0mL

Alkaline hematin solution............................. 6.25mL

Antibiotic Solution:

Composition per 10.0mL:

Rifampin .........................................................0.025g

Cefsulodin......................................................6.25mg

Polymyxin B sulfate ....................................20,000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Alkaline Hematin Solution:

Composition per 10.0mL:

Hemin .............................................................0.032g

NaOH (0.15N solution)................................. 10.0mL

Preparation of Alkaline Hematin Solution:

Add hemin to 10.0mL of NaOH solution. Mix thor

oughly. Autoclave for 30 min at 5 psi pressure–108°C.

Cool to 25°C.

Preparation of Medium: Add components, ex

cept antibiotic solution and alkaline hematin solu

tion, to distilled/deionized water and bring volume to

983.75mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 10.0mL

of sterile antibiotic solution and 6.25mL of sterile al

kaline hematin solution. Mix thoroughly. Aseptically

distribute into sterile tubes. Use medium immediate

ly or store overnight at 4°C.

Use: For the enrichment of Campylobacter species

from foods.