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Wickerham Broth

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wickerham Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

KNO3 ................................................................0.78g

Yeast carbon base........................................ 100.0mL

pH 4.5 ± 0.2 at 25°C

Yeast Carbon Base:

Composition per liter:

Glucose .............................................................10.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O ........................................................0.1g

DL-Methionine...................................................0.02g

DL-Tryptophan...................................................0.02g

L-Histidine·HCl .................................................0.01g

Inositol .............................................................2.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyridoxine........................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

KI .....................................................................0.1mg

CuSO4·5H2O ..................................................0.04mg

Folic acid.......................................................... 2.0μg

Biotin ............................................................... 2.0μg

Preparation of Yeast Carbon Base: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast

carbon base, add 0.78g of KNO3 (or peptone). Mix

thoroughly. Filter sterilize. Aseptically distribute

0.5mL into tubes containing 4.5mL of sterile dis

tilled/deionized water.

Use: For the cultivation and differentiation of bacte

ria based on nitrate assimilation.