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Wilson Blair Base

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wilson Blair Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................30.0g

Proteose peptone No. 3.....................................10.0g

Glucose .............................................................10.0g

Beef extract.........................................................5.0g

NaCl....................................................................5.0g

Selective reagent........................................... 70.0mL

Brilliant Green (1% solution) ......................... 4.0mL

pH 7.3 ± 0.2 at 25°C

Selective Reagent:

Composition per 100.0mL:

Solution 1.................................................... 100.0mL

Solution 2.................................................... 100.0mL

Solution 3.................................................... 100.0mL

Solution 4...................................................... 20.2mL

Preparation of Selective Reagent: Combine

100.0mL of solution 1, 100.0mL of solution 2,

100.0mL of solution 3, and 20.2mL of solution 4.

Mix thoroughly. Gently heat to boiling until a slate

grey color develops. Cool to 50°C.

Solution 1:

Composition per 100.0mL:

NaHSO3 ............................................................40.0g

Preparation of Solution 1: Add NaHSO3 to

100.0mL of distilled/deionized water. Mix thorough

ly.

Solution 2:

Composition per 100.0mL:

NaH2PO4 ...........................................................21.0g

Preparation of Solution 2: Add NaH2PO4 to

100.0mL of distilled/deionized water. Mix thorough

ly.

Solution 3:

Composition per 100.0mL:

Bismuth ammonium citrate...............................12.5g

Preparation of Solution 3: Add bismuth ammo

nium citrate to 100.0mL of distilled/deionized water.

Mix thoroughly.

Solution 4:

Composition per 20.2mL:

FeSO4 ................................................................0.96g

Preparation of Solution 4: Add FeSO4 to

20.0mL of distilled/deionized water. Add 0.2mL of

concentrated HCl. Mix thoroughly.

Preparation of Medium: Add components, ex

cept selective reagent and Brilliant Green solution, to

distilled/deionized water and bring volume to

976.0mL. Mix thoroughly. Gently heat and bring to

boiling. Distribute into tubes or flasks. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add selective reagent and Brilliant Green

solution. Mix thoroughly. Pour into sterile Petri dish

es or leave in tubes.

Use: For the isolation and cultivation of Salmonella,

especially Salmonella typhi.

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Wilson Blair Base