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WL Medium with Tomato Juice (Wallerstein Laboratory Medium with Tomato Juice)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

WL Medium with Tomato Juice

(Wallerstein Laboratory Medium

with Tomato Juice)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................50.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................4.0g

KH2PO4.............................................................0.55g

KCl..................................................................0.425g

CaCl2...............................................................0.125g

MgSO4·7H2O ..................................................0.125g

Bromcresol Green ...........................................0.022g

FeCl3 ................................................................2.5mg

MnSO4·4H2O...................................................2.5mg

Tomato juice, canned, clarified................... 400.0mL

pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of microorganisms from al

coholic mash.