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WL Nutrient Agar (Wallerstein Laboratory Nutrient Agar)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

WL Nutrient Agar

(Wallerstein Laboratory Nutrient Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................50.0g

Agar ..................................................................20.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................4.0g

KH2PO4.............................................................0.55g

KCl..................................................................0.425g

CaCl2·2H2O ....................................................0.125g

MgSO4·7H2O..................................................0.125g

Bromcresol Green ...........................................0.022g

FeCl3 ................................................................2.5mg

MnSO4·4H2O ...................................................2.5mg

Na2CO3 (1% solution) .................................. 30.0mL

pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat with mixing and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the detection of bacteria and yeasts from in

dustrial fermentation processes, particularly from

beer processing.