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XLD Agar (Xylose Lysine Deoxycholate Agar) (BAM M179)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

XLD Agar

(Xylose Lysine Deoxycholate Agar)

(BAM M179)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Lactose................................................................7.5g

Sucrose................................................................7.5g

Na2S2O3 ..............................................................6.8g

L-Lysine...............................................................5.0g

NaCl....................................................................5.0g

Xylose ...............................................................3.75g

Yeast extract........................................................3.0g

Sodium deoxycholate..........................................2.5g

Ferric ammonium citrate.....................................0.8g

Phenol Red........................................................0.08g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not overheat. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes. Plates should be

poured as soon as possible to avoid precipitation.

Use: For the isolation and differentiation of enteric

pathogens, especially Shigella and Providencia spe

cies. Nonfermenting xylose/lactose/sucrose bacteria

appear as red colonies. Xylose-fermenting, lysine

decarboxylating bacteria appear as red colonies. Xy

lose-fermenting, lysine-nondecarboxylating bacteria

appear as opaque yellow colonies. Lactose or su

crose-fermenting bacteria appear as yellow colonies.