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Yersinia Selective Agar Base

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Mannitol............................................................20.0g

Peptone..............................................................17.0g

Agar ..................................................................12.5g

Proteose peptone.................................................3.0g

Yeast extract........................................................2.0g

Sodium pyruvate.................................................2.0g

NaCl....................................................................1.0g

Sodium deoxycholate.........................................0.5g

MgSO4·7H2O ....................................................0.01g

Neutral Red.......................................................0.03g

Crystal Violet ...................................................1.0mg

Selective supplement ...................................... 6.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Selective Supplement:

Composition per 6.0mL:

Cefsulodin......................................................15.0mg

Irgasan..............................................................4.0mg

Novobiocin.......................................................2.5mg

Ethanol............................................................ 2.0mL

Preparation of Selective Supplement: Asepti

cally add components to 4.0mL of distilled/deionized

water and 2.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°C. Aseptically

add selective supplement. Mix thoroughly. Pour into

sterile Petri dishes or leave in tubes.

Use: For the isolation and enumeration of Yersinia en

terocolitica from foods.