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Z-Agar

[所属分类:培养基配方] [发布时间:2021-12-30] [发布人:网站管理员2] [阅读次数:] [返回]
Z-Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ........................................................... 16.0
K2HPO4...............................................................5.0g
K2SO4..................................................................2.0g
KH2PO4...............................................................1.0g
MgSO4·7H2O ....................................................0.05g
Acetamide solution ....................................... 40.0mL
pH 7.2 ± 0.2 at 25°C
Acetamide Solution:
Composition per 100.0mL:
Acetamide .........................................................10.0g
Preparation of Acetamide Solution: Add aceta
mide to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept acetamide solution, to distilled/deionized water
and bring volume to 960.0mL. Mix thoroughly. Au
toclave for 20 min at 15 psi pressure–121°C. Cool to
70°C. Aseptically add 40.0mL sterile acetamide so
lution. Mix thoroughly. Pour into sterile Petri dishes.
Dry plates at 37°C for 30 min.
Use: For the isolation of Pseudomonas aeruginosa
from milk.
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