Aquabacter spiritensis Medium (LMG Medium 225)

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Aquabacter spiritensis Medium
(LMG Medium 225)
Sodium succinate..............................................................2.0g
Yeast extract..................................................................1.0g
KH2PO4 .........................................................................1.0g
Peptone.........................................................................0.4g
NH4Cl ...........................................................................0.2g
NaCl..............................................................................0.2g
MgSO4·7H2O......................................................................0.2g
CaCl2·2H2O.......................................................................10.0mg
Ferric citrate...................................................................5.0mg
Vitamin solution..................................................................20.0mL
Trace elements solution SL-6 ...................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution SL-6 :
Composition per liter:
H3BO3 ..............................................................................0.3g
CoCl2 ·6H2O ........................................................................0.2g
ZnSO4 ·7H2O..........................................................................0.1g
MnCl2 ·4H2O.........................................................................0.03g
Na2MoO4·H2O .........................................................................0.03g
NiCl2 ·6H2O..........................................................................0.02g
CuCl2· 2H2O..........................................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 3.4.
Vitamin Solution:
Composition per liter:
Calcium  DL -pantothenate...........................................................5.0mg
Riboflavin ..........................................................................5.0mg
Thiamine·HCl .......................................................................5.0mg
Biotin ..............................................................................2.0mg
Folic acid...........................................................................2.0mg
Vitamin B 12 ........................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to 980.0mL distilled/deionized water. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add
20.0mL sterile vitamin solution. Mix thoroughly. Aseptically distribute
to sterile tubes or flasks.
Use: For the cultivation of Aquabacter spiritensis.