ASW Medium (Artificial Seawater Medium)

[所属分类:培养基配方] [发布时间:2019-11-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ASW Medium
(Artificial Seawater Medium)
Composition per liter:
NaCl............................................................................................27.0g
Agar............................................................................................15.0g
MgSO4 ·7H2O................................................................................6.6g
MgCl2 ·6H2O .................................................................................5.6g
CaCl2 ·2H2O ..................................................................................1.5g
KNO3 ............................................................................................1.0g
KH2PO4 .......................................................................................0.07g
NaHCO3 ......................................................................................0.04g
Tris-HCl buffer (1.0M, pH 7.6)................................................20.0mL
Chelated iron solution................................................................1.0mL
Trace metal solution...................................................................1.0mL
Trace Metal Solution:
Composition per 100.0mL:
H3BO3 ......................................................................................60.0mg
MnCl2 ·4H2O.............................................................................40.0mg
(NH4)6Mo7O24·4H2O...............................................................37.0mg
CuCl2 ·2H2O ...............................................................................4.0mg
ZnCl2 ..........................................................................................4.0mg
CoCl2 ·6H2O ...............................................................................1.5mg
Preparation of Trace Metal Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Chelated Iron Solution:
Composition per 100.0mL:
FeCl3 ·4H2O............................................................................240.0mg
EDTA..........................................................................................14.6g
Preparation of Chelated Iron Solution: Add EDTA to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust
pH to 7.6. Add FeCl 3 ·4H 2 O. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Porphyridium purpureum.