ATS Medium (American Trudeau Society Medium)

[所属分类:培养基配方] [发布时间:2019-11-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ATS Medium
(American Trudeau Society Medium)
Composition per liter:
Potato..........................................................................................20.0g
Malachite Green............................................................................0.2g
Egg yolk emulsion.................................................................500.0mL
Glycerol ...................................................................................10.0mL
pH 6.5–7.0 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Distribute into tubes. Autoclave for 15
min at 15 psi pressure–121°C in a slanted position.
Use: For the isolation and cultivation of Mycobacterium species other
than Mycobacterium leprae. Especially useful for the detection of
Mycobacterium tuberculosis from clinical specimens such as cerebro-
spinal fluid, pleural fluid, and tissues.