Autotrophic Nitrobacter Medium (LMG Medium 247)

[所属分类:培养基配方] [发布时间:2019-11-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Autotrophic Nitrobacter Medium
(LMG Medium 247)
Composition per liter:
NaNO2 ...........................................................................................2.0g
Stock solution.........................................................................100.0mL
Trace elements solution .............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH2PO4 .........................................................................................1.5g
MgSO4 ·7H2O................................................................................0.5g
CaCO3 .........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
(NH4)Mo7O2 ........................................................................437.10mg
FeSO4 ·7H2O...........................................................................97.30mg
ZnSO4 ·7H2O..........................................................................43.10mg
H3BO3 ....................................................................................39.40mg
MnSO4 ·H2O...........................................................................33.80mg
CuSO2 ·5H2O..........................................................................25.00mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.6 with
NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Allow the medium to stand for 2–3 days so that the
pH can adjust itself to pH 7.4–7.6.
Use: For the cultivation of autotrophic Nitrobacter spp.