AV Agar with Vitamins

[所属分类:培养基配方] [发布时间:2019-11-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AV Agar with Vitamins
Composition per liter:
Agar............................................................................................15.0g
Glucose.........................................................................................1.0g
Glycerol ........................................................................................1.0g
L- Arginine.....................................................................................0.3g
K2HPO4 .........................................................................................0.3g
NaCl..............................................................................................0.3g
MgSO4 ·7H2O................................................................................0.2g
Vitamin solution.....................................................................100.0mL
Trace salts solution ....................................................................1.0mL
Vitamin Solution:
Composition per 100.0mL:
p-Aminobenzoic acid.................................................................0.5mg
Calcium pantothenate ................................................................0.5mg
HCl.............................................................................................0.5mg
Inositol.......................................................................................0.5mg
Niacin.........................................................................................0.5mg
Pyridoxine..................................................................................0.5mg
Riboflavin..................................................................................0.5mg
Thiamine·HCl ............................................................................0.5mg
Biotin.......................................................................................0.25mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Trace Salts Solution:
Composition per liter:
FeSO4 ·7H2O................................................................................10.0g
CuSO4 ·5H2O.................................................................................1.0g
MnSO4 ·7H2O................................................................................1.0g
ZnSO4 ·7H2O.................................................................................1.0g
Preparation of Trace Salts Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of
sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation and cultivation of Actinomadura species, Acti-
nopolyspora species, Excellospora species, and Microspora species.