Azospirillum amazonense Medium (LGI Medium)

[所属分类:培养基配方] [发布时间:2019-11-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azospirillum amazonense Medium
(LGI Medium)
Composition per liter:
Sucrose..................................................................5.0g
Agar....................................................................1.75g
KH2PO4 ..................................................................0.6g
K2HPO4 ..................................................................0.2g
MgSO4 ·7H2O.............................................................0.2g
CaCl2 ·2H2O ............................................................0.02g
FeCl3 ...................................................................0.01g
Na2MoO4 ·2H2O...........................................................2.0mg
Bromthymol Blue solution ................................................5.0mL
pH 6.0 ± 0.2 at 25°C
Bromthymol Blue Solution:
Composition per 100.0mL:
Bromthymol Blue ........................................................0.5g
Preparation of Bromthymol Blue Solution:  Add Bromthymol
Blue to 100.0mL of 0.2N KOH. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Azospirillum amazon-
ense.