Azotobacter Medium

[所属分类:培养基配方] [发布时间:2019-11-21] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Medium
Composition per liter:
Agar..........................................................................15.0g
CaCO3 .........................................................................5.0g
K2HPO4 ........................................................................0.9g
CaCl2 ·2H2O ..................................................................0.1g
KH2PO4 ........................................................................0.1g
MgSO4 ·7H2O...................................................................0.1g
FeSO4 ·7H2O..................................................................0.01g
Na2MoO4 ·2H2O................................................................5.0mg
Glucose solution ............................................................25.0mL
Mannitol solution.............................................................25.0mL
pH 7.3 ± 0.2 at 25°C
Glucose Solution:
Composition per 25.0mL:
D- Glucose......................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 25.0mL. Mix thoroughly. Filter steril-
ize. Warm to 50°–55°C.
Mannitol Solution:
Composition per 25.0mL:
Mannitol.........................................................................5.0g
Preparation of Mannitol Solution : Add mannitol to distilled/de-
ionized water and bring volume to 25.0mL. Mix thoroughly. Filter ster-
ilize. Warm to 50°–55°C.
Preparation of Medium: Add components, except glucose solu-
tion and mannitol solution, to distilled/deionized water and bring vol-
ume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 25.0mL of sterile
glucose solution and 25.0mL of sterile mannitol solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Azotobacter species.