Azotobacter Supplement (ATCC Medium 15)

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter Supplement
(ATCC Medium 15)
Composition per liter:
Agar................................................................................15.0g
K2HPO4 ..............................................................................1.0g
MgSO4 ·7H2O.........................................................................0.2g
NaCl.................................................................................0.2g
FeSO4 ·7H2O.......................................................................5.0mg
Soil extract......................................................................100.0mL
Mannitol solution.................................................................100.0mL
pH 6.0 ± 0.2 at 25°C
Soil Extract:
Composition per 200.0mL:
African Violet soil...................................................................0.5g
Na2CO3 ...............................................................................0.5g
Preparation of Soil Extract: Add components to distilled/deion-
ized water and bring volume to 200.0mL. Autoclave for 60 min at 15
psi pressure–121°C. Filter through Whatman filter paper.
Mannitol Solution:
Composition per 100.0mL:
Mannitol.............................................................................20.0g
Preparation of Mannitol Solution:  Add mannitol to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except mannitol solu-
tion, to tap water and bring volume to 900.0mL. Mix thoroughly. Ad-
just pH to 6.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C. Aseptically add 100.0mL of sterile mannitol solution. Mix
thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Azomonas macrocytogenes.