Azotobacter vinelandii Medium

[所属分类:培养基配方] [发布时间:2019-11-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Azotobacter vinelandii Medium
Composition per liter:
Sodium benzoate...............................................................1.0g
K2HPO4 .......................................................................0.5g
Ethanol......................................................................1.0mL
Preparation of Medium: Add components, except ethanol, to dis-
tilled/deionized water and bring volume to 999.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 1.0mL of filter-sterilized ethanol. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Azotobacter vinelandii from soil.